Which reagent is used to detect the presence of protein?
Sudan IV
Benedict's
Biuret
Iodine
The Correct Answer is C
Choice A rationale: Sudan IV is not a reagent for protein detection, but a reagent for lipid detection. Sudan IV is a red dye that binds to non-polar molecules, such as fats and oils. Sudan IV stains lipids red, while leaving water-soluble molecules, such as proteins, unstained¹.
Choice B rationale: Benedict's is not a reagent for protein detection, but a reagent for sugar detection. Benedict's is a blue solution that contains copper sulfate, sodium carbonate, and sodium citrate. Benedict's reacts with reducing sugars, such as glucose and fructose, and reduces the copper ions from blue to orange-red².
Choice C rationale: Biuret is a reagent for protein detection. Biuret is a blue solution that contains copper sulfate and sodium hydroxide. Biuret reacts with peptide bonds, which are the bonds that link amino acids in proteins. Biuret changes color from blue to violet when it binds to protein molecules³.
Choice D rationale: Iodine is not a reagent for protein detection, but a reagent for starch detection. Iodine is a brown solution that forms a complex with starch, a polysaccharide composed of glucose units. Iodine changes color from brown to blue-black when it interacts with starch molecules⁴.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is C
Explanation
Choice A rationale: Eukaryotes is incorrect because eukaryotes are organisms that have cells with a nucleus and other membrane-bound organelles, such as animals, plants, fungi, and protists. Some eukaryotes, such as protists, are microscopic and require microscopy to be studied, but others, such as animals and plants, are macroscopic and can be seen with the naked eye.
Choice B rationale: Fungi is incorrect because fungi are a group of eukaryotic organisms that include mushrooms, molds, yeasts, and lichens. Some fungi, such as yeasts and molds, are microscopic and require microscopy to be studied, but others, such as mushrooms and lichens, are macroscopic and can be seen with the naked eye.
Choice C rationale: Prokaryotes is correct because prokaryotes are organisms that have cells without a nucleus or other membrane-bound organelles, such as bacteria and archaea. All prokaryotes are microscopic and require microscopy to be studied. Prokaryotes are the smallest and most abundant living organisms on Earth.
Choice D rationale: Plants is incorrect because plants are a group of eukaryotic organisms that have cells with a cell wall, chloroplasts, and a large central vacuole, such as mosses, ferns, and flowering plants. Some plants, such as mosses and algae, are microscopic and require microscopy to be studied, but others, such as ferns and flowering plants, are macroscopic and can be seen with the naked eye.
Correct Answer is A
Explanation
Choice A rationale: A hypertonic solution has a higher solute concentration than the potato cell, which means it has a lower water potential. Water will move out of the potato cell by osmosis, causing it to shrink and become soft and limp.
Choice B rationale: A hypotonic solution has a lower solute concentration than the potato cell, which means it has a higher water potential. Water will move into the potato cell by osmosis, causing it to swell and become turgid and firm.
Choice C rationale: An isotonic solution has the same solute concentration as the potato cell, which means it has the same water potential. Water will move in and out of the potato cell at the same rate, causing it to remain unchanged in size and shape.
Choice D rationale: Tonic is not a valid term to describe the solute concentration of a solution. The correct terms are hypertonic, hypotonic, or isotonic.
Choice E rationale: I cannot determine anything without comparing multiple solutions is incorrect because the appearance of the potato slice after soaking in the solution provides enough information to determine the relative solute concentration of the solution.
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