Which of the following is NOT a product of the light reaction?

Choice A reason: Biuret test is a test for the presence of proteins or peptides in a solution. It involves adding Biuret reagent, which is a blue solution of copper (II) sulfate and sodium hydroxide, to the solution. If proteins or peptides are present, they form a complex with the copper (II) ions, which changes the color of the solution to violet or pink. ³

Choice B reason: Gram's iodine test is a test for the presence of starch in a solution. It involves adding iodine solution, which is a brown solution of iodine and potassium iodide, to the solution. If starch is present, it forms a complex with the iodine molecules, which changes the color of the solution to blue-black. ⁴

Choice C reason: Ninhydrin test is a test for the presence of amines or amino acids in a solution. It involves adding ninhydrin reagent, which is a purple solution of ninhydrin, to the solution. If amines or amino acids are present, they react with ninhydrin to produce a purple color, often called Ruhemann's purple. ¹

Choice D reason: Brown paper test is a test for the presence of lipids, such as fats or oils, in a solution. The test involves placing a drop of the solution on a piece of brown paper and letting it dry. If lipids are present, they leave a translucent spot on the paper, which can be seen by holding the paper against a light source. The test is based on the fact that lipids are nonpolar and do not dissolve in water, but can dissolve in organic solvents and stain the paper. ²

Choice E reason: Benedict's test is a test for the presence of reducing sugars, such as glucose or maltose, in a solution. The test involves adding Benedict's reagent, which is a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, to the solution and heating it in a water bath. If reducing sugars are present, they reduce the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. ⁵

Choice A rationale: Cell membranes pressed tightly against the cell walls is correct because this is what happens when a plant cell is placed in a hypotonic solution. A hypotonic solution has a higher concentration of water than the cell, so water moves into the cell and out of the solution by osmosis, causing the cell to swell and press against the cell wall. This is called turgor and it helps the cell maintain its shape and rigidity.

Choice B rationale: Many of the cells had burst is incorrect because plant cells do not burst in a hypotonic solution, unlike animal cells. Plant cells have a rigid cell wall that prevents them from bursting, even when they are full of water. The cell wall can withstand the pressure of water entering the cell.

Choice C rationale: Full central vacuoles is correct because this is also what happens when a plant cell is placed in a hypotonic solution. The central vacuole is a large organelle that stores water and other substances in the plant cell. When water enters the cell, the central vacuole expands and fills up the cell, increasing its turgor pressure.

Choice D rationale: Many of the cells had become crenated is incorrect because crenation is the opposite of what happens in a hypotonic solution. Crenation is the process by which a cell shrinks and becomes wrinkled due to water loss in a hypertonic solution. A hypertonic solution has a lower concentration of water than the cell, so water moves out of the cell and into the solution by osmosis, causing the cell to shrink.