What is the purpose of using PCR (polymerase chain reaction) in the laboratory?
To separate DNA fragments by size.
To amplify specific regions of DNA.
To sequence DNA fragments.
To analyze protein expression levels.
The Correct Answer is B
PCR (polymerase chain reaction) is a laboratory technique used to make many copies of a specific region of DNA.
The goal of PCR is to make enough of the target DNA region that it can be analyzed or used in some other way.
PCR has many research and practical applications, including DNA cloning, medical diagnostics, and forensic analysis of DNA.
Choice A is incorrect because PCR does not separate DNA fragments by size. Choice C is incorrect because PCR does not sequence DNA fragments.
Choice D is incorrect because PCR does not analyze protein expression levels.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
The terminal velocity of an object falling through a fluid is affected by several factors, including its mass and shape.
Objects with large surface areas will often experience a large amount of air resistance when they move.
Choice B is incorrect because the volume of the object does not affect its terminal velocity.
Choice C is incorrect because the acceleration and momentum of the object do not affect its terminal velocity.
Choice D is incorrect because the height and distance of the fall do not affect the terminal velocity of a falling object.
Correct Answer is C
Explanation
A primer is a short single-stranded DNA fragment used in certain laboratory techniques, such as the polymerase chain reaction (PCR).
In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified.
Choice A) A short piece of double-stranded DNA that binds to the template DNA and acts as a “starter” for the polymerase is incorrect because primers are single-stranded, not double-stranded.
Choice B) A short piece of double-stranded DNA that binds to the primer and acts as a “starter” for the template is incorrect because it does not make sense for a primer to bind to itself.
Choice D) A short piece of single-stranded DNA that binds to the polymerase and acts as a “starter” for the template is incorrect because primers bind to the template DNA, not to the polymerase.
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