What is the purpose of using PCR (polymerase chain reaction) in the laboratory?
To separate DNA fragments by size.
To amplify specific regions of DNA.
To sequence DNA fragments.
To analyze protein expression levels.
The Correct Answer is B
PCR (polymerase chain reaction) is a laboratory technique used to make many copies of a specific region of DNA.
The goal of PCR is to make enough of the target DNA region that it can be analyzed or used in some other way.
PCR has many research and practical applications, including DNA cloning, medical diagnostics, and forensic analysis of DNA.
Choice A is incorrect because PCR does not separate DNA fragments by size. Choice C is incorrect because PCR does not sequence DNA fragments.
Choice D is incorrect because PCR does not analyze protein expression levels.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is C
Explanation
Estrogen is a steroid hormone responsible for the development of female secondary sexual characteristics such as breast development, menstrual cycle, and widening of hips.
Choice A, Progesterone, is not the correct answer because it is required to maintain pregnancy and delivery.
Choice B, Testosterone, is not the correct answer because it is a hormone produced by the testes which control the development of male secondary sexual characteristics.
Choice D, FSH (Follicle Stimulating Hormone), is not the correct answer because it stimulates the growth of ovarian follicles in females and regulates spermatogenesis in males.
Correct Answer is C
Explanation
Granzymes are a family of serine proteases that are stored in and secreted from the cytotoxic granules of cytotoxic T lymphocytes (CTL) and natural killer (NK) cells.
They work in synergy with perforin, a pore-forming toxin, to induce apoptosis in target cells.
Perforin is necessary for the delivery of granzyme B to the target cell cytosol where caspase-dependent and -independent pathways to apoptosis are activated.
Perforins (choice A) are pore-forming toxins that work in synergy with granzymes to induce apoptosis in target cells.
Cytokines (choice B) are signaling molecules that regulate immune responses but do not directly induce apoptosis in target cells.
Interferons (choice D) are a type of cytokine that play a role in immune responses but do not directly induce apoptosis in target cells.
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