Which reagent is used to detect the presence of starch?
Iodine
Biuret
Benedict's
Phenol red
Sudan IV
The Correct Answer is A
Choice A rationale: Iodine is a chemical element that forms a brown solution of iodine and potassium iodide, known as iodine solution. When this solution is added to a sample that contains starch, it forms a complex with the starch molecules, which changes the color of the solution to blue-black. This is based on the fact that starch is a polysaccharide that has a helical structure, which can trap the iodine molecules inside. ²
Choice B rationale: Biuret is a chemical compound that forms a blue solution of copper (II) sulfate and sodium hydroxide, known as biuret reagent. When this reagent is added to a sample that contains proteins or peptides, it forms a complex with the copper (II) ions, which changes the color of the solution to violet or pink. This is based on the fact that proteins and peptides have peptide bonds, which have nitrogen atoms that can coordinate with the copper (II) ions. ³
Choice C rationale: Benedict's is a chemical compound that forms a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, known as Benedict's reagent. When this reagent is heated with a sample that contains reducing sugars, such as glucose or maltose, it reduces the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. This is based on the fact that reducing sugars have free aldehyde or ketone groups that can donate electrons to the copper (II) ions. ⁴
Choice D rationale: Phenol red is a chemical compound that forms a red solution that is used as a pH indicator. When this solution is added to a sample that has an acidic or neutral pH, it remains red or turns yellow. When this solution is added to a sample that has an alkaline pH, it turns pink or fuchsia. This is based on the fact that phenol red has a sulfonated hydroxyquinone group that can lose or gain protons depending on the pH of the solution. ⁵
Choice E rationale: Sudan IV is a chemical compound that forms a red powder that is used as a stain for lipids. When this powder is dissolved in a solvent and added to a sample that contains lipids, such as fats or oils, it dissolves in the lipids and stains them red. When this solution is added to a sample that does not contain lipids, it remains in the solvent and does not stain the sample. This is based on the fact that Sudan IV is a nonpolar compound that can dissolve in nonpolar substances like lipids, but not in polar substances like water. ⁶.
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Naxlex Comprehensive Predictor Exams
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Correct Answer is A
Explanation
Choice A reason: Benedict's test is a test for the presence of reducing sugars, such as glucose or maltose, in a solution. The test involves adding Benedict's reagent, which is a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, to the solution and heating it in a water bath. If reducing sugars are present, they reduce the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. The color and amount of the precipitate indicate the concentration of reducing sugars in the solution. ¹
Choice B reason: Brown paper test is a test for the presence of lipids, such as fats or oils, in a solution. The test involves placing a drop of the solution on a piece of brown paper and letting it dry. If lipids are present, they leave a translucent spot on the paper, which can be seen by holding the paper against a light source. The test is based on the fact that lipids are nonpolar and do not dissolve in water, but can dissolve in organic solvents and stain the paper. ²
Choice C reason: Biuret test is a test for the presence of proteins or peptides in a solution. The test involves adding Biuret reagent, which is a blue solution of copper (II) sulfate and sodium hydroxide, to the solution. If proteins or peptides are present, they form a complex with the copper (II) ions, which changes the color of the solution to violet or pink. The test is based on the fact that proteins and peptides have peptide bonds, which have nitrogen atoms that can coordinate with the copper (II) ions. ³
Choice D reason: Iodine test is a test for the presence of starch in a solution. The test involves adding iodine solution, which is a brown solution of iodine and potassium iodide, to the solution. If starch is present, it forms a complex with the iodine molecules, which changes the color of the solution to blue-black. The test is based on the fact that starch is a polysaccharide that has a helical structure, which can trap the iodine molecules inside. ⁴
Choice E reason: Wendelspecht test is a fictional test that does not exist in reality. It is a made-up name that has no meaning or relevance to the topic of this question. Therefore, it cannot be a valid answer.
Correct Answer is B
Explanation
Choice A rationale: Plant pigments do not produce photon energy, but rather capture it from the sun. Photon energy is the energy carried by particles of light, called photons. Different types of electromagnetic radiation, such as visible light, have different amounts of photon energy depending on their wavelength¹.
Choice B rationale: Plant pigments absorb light energy and use it to initiate photosynthesis. Photosynthesis is the process by which plants convert light energy into chemical energy, stored in the bonds of sugar molecules. Plant pigments are specialized organic molecules, such as chlorophyll and carotenoids, that are found in the chloroplasts of plant cells. They absorb specific wavelengths of light and reflect others, giving plants their characteristic colors²³.
Choice C rationale: Plant pigments do not provide electrons, but rather transfer them to other molecules. Electrons are negatively charged subatomic particles that are involved in chemical reactions. In photosynthesis, plant pigments absorb light energy and use it to split water molecules, releasing electrons, protons, and oxygen. The electrons are then passed along an electron transport chain, generating a proton gradient that drives the synthesis of ATP, an energy molecule. The electrons are also used to reduce NADP+ to NADPH, an electron carrier⁴.
Choice D rationale: Plant pigments do not convert heat to electricity, but rather convert light to chemical energy. Heat and electricity are both forms of energy, but they are not directly involved in photosynthesis. Heat is the kinetic energy of molecules, while electricity is the flow of electrons or electric charge. Plant pigments absorb light energy and use it to drive the chemical reactions of photosynthesis, which produce sugar and oxygen as products⁵.
Choice E rationale: Plant pigments do not reduce NADP, but rather donate electrons to it. Reduction is a chemical reaction in which a molecule gains electrons, while oxidation is a chemical reaction in which a molecule loses electrons. NADP+ is an oxidized form of NADP, which stands for nicotinamide adenine dinucleotide phosphate. It is an electron carrier that accepts electrons from plant pigments in photosystem I, a complex of proteins and pigments in the thylakoid membrane of the chloroplast. The reduced form of NADP is NADPH, which carries electrons and hydrogen for the dark reaction of photosynthesis, which uses CO2 to produce glucose⁶.
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