Which objective lens provides the highest total magnification?

Choice A rationale: Distilled water is not a positive control, but a negative control for the iodine test. Distilled water is a pure solvent that does not contain any starch or other carbohydrates. It does not react with the iodine solution and does not produce any color change. A negative control is used to confirm that there is no response to the reagent or the microorganism used in the test. It is used to set the baseline and verify that the detecting reagent is working properly³.

Choice B rationale: Olive oil is not a positive control, but a negative control for the iodine test. Olive oil is a lipid that does not contain any starch or other carbohydrates. It does not react with the iodine solution and does not produce any color change. A negative control is used to confirm that there is no response to the reagent or the microorganism used in the test. It is used to set the baseline and verify that the detecting reagent is working properly³.

Choice C rationale: Albumin solution is not a positive control, but a negative control for the iodine test. Albumin is a protein that does not contain any starch or other carbohydrates. It does not react with the iodine solution and does not produce any color change. A negative control is used to confirm that there is no response to the reagent or the microorganism used in the test. It is used to set the baseline and verify that the detecting reagent is working properly³.

Choice D rationale: Starch solution is a positive control for the iodine test. Starch is a polysaccharide that contains many glucose units linked by glycosidic bonds. Starch reacts with the iodine solution and produces a blue-black color. A positive control is used to confirm that the test works and gives a positive result when the substance is present³.

Choice E rationale: Glucose solution is not a positive control, but a negative control for the iodine test. Glucose is a monosaccharide that does not contain any glycosidic bonds. Glucose does not react with the iodine solution and does not produce any color change. A negative control is used to confirm that there is no response to the reagent or the microorganism used in the test. It is used to set the baseline and verify that the detecting reagent is working properly³.

Choice A rationale: High power is incorrect because high power is the second highest magnification objective lens, not the highest. High power is also called the 40x objective lens because it magnifies the specimen by 40 times. When combined with the 10x eyepiece lens, the total magnification is 400x.

Choice B rationale: Oil immersion is correct because oil immersion is the highest magnification objective lens. Oil immersion is also called the 100x objective lens because it magnifies the specimen by 100 times. When combined with the 10x eyepiece lens, the total magnification is 1000x. Oil immersion requires oil to be applied between the slide and the lens to reduce the refraction of light and increase the clarity of the image.

Choice C rationale: Low power is incorrect because low power is the second lowest magnification objective lens, not the highest. Low power is also called the 10x objective lens because it magnifies the specimen by 10 times. When combined with the 10x eyepiece lens, the total magnification is 100x.

Choice D rationale: Scanning is incorrect because scanning is the lowest magnification objective lens, not the highest. Scanning is also called the 4x objective lens because it magnifies the specimen by 4 times. When combined with the 10x eyepiece lens, the total magnification is 40x. Scanning is used to scan the whole slide and find the specimen.