To separate genomic DNA fragments by size, which of these laboratory methods is most useful?
Titration.
Electrophoresis.
Filtration.
Spectrophotometry.
The Correct Answer is B
Electrophoresis is a technique that uses an electric field to separate charged molecules, such as DNA fragments, based on their size and charge.
Choice A is not correct because titration is a laboratory method used to determine the concentration of a solution.
Choice C is not correct because filtration is a laboratory method used to separate solids from liquids.
Choice D is not correct because spectrophotometry is a laboratory method used to measure the absorbance of light by a solution.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
The glomerulus is the main filtering unit of the kidney.
It is formed by a network of small blood vessels (capillaries) enclosed within a sac called the Bowman’s capsule.
The blood supply to the glomerulus is provided via the afferent arteriole.
The blood then flows through the capillary network, where it gets filtered, and then leaves the glomerulus via the efferent arteriole.
Choice B. Proximal tubule is not correct because it is where the ultrafiltrate collected in the Bowman’s space drains directly into.
Choice C. Distal tubule is not correct because it is not mentioned in relation to blood exiting the glomerulus.
Choice D. Afferent arteriole is not correct because it provides blood supply to the glomerulus.
Correct Answer is B
Explanation
The correct answer is choice B. 3’ TCGATCGCA 5’.
In DNA, the nitrogenous bases adenine (A) and thymine (T) pair together, while cytosine © and guanine (G) pair together.
Therefore, the complementary strand of the given DNA sequence “5’ AGCTAGCGT 3’” would have the nitrogenous bases T, C, G, A, T, C, G, C and A in that order.
Additionally, the 5’ to 3’ direction of the complementary strand is opposite to that of the given strand.
Choice A. 3’ AGCTAGCGT 5’ is not correct because it is not complementary to the given strand.
Choice C. 5’ UCGAUCGCA 3’ is not correct because it contains uracil (U), which is a nitrogenous base found in RNA, not DNA.
Choice D. 3’ TCGUTCGCU 3’ is not correct because it also contains uracil (U), which is a nitrogenous base found in RNA, not DNA.
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