Which of the following items will you need to make a wet mount?
Check all that apply.
Transfer pipette
Clean slide
Bunsen burner
Timer
Cover slip
Correct Answer : A,B,E,F
Choice A rationale: Transfer pipette is correct because you need a transfer pipette to transfer a drop of water or a liquid specimen onto the center of the slide. A transfer pipette is a plastic or glass tube with a bulb at one end that can suck up and release liquids.
Choice B rationale: Clean slide is correct because you need a clean slide to place the specimen on. A slide is a thin, flat piece of glass or plastic that can be viewed under a microscope. A clean slide ensures that there is no contamination or interference from other substances.
Choice C rationale: Bunsen burner is incorrect because you do not need a Bunsen burner to make a wet mount. A Bunsen burner is a device that produces a flame by mixing gas and air. A Bunsen burner is used for heating, sterilizing, or burning substances, not for making a wet mount.
Choice D rationale: Timer is incorrect because you do not need a timer to make a wet mount. A timer is a device that measures or indicates the passage of time. A timer is used for timing experiments, not for making a wet mount.
Choice E rationale: Tweezers is correct because you need tweezers to pick up and place the specimen on the slide. Tweezers are a pair of metal or plastic pincers that can grasp and hold small objects. Tweezers are useful for handling delicate or microscopic specimens.
Choice F rationale: Cover slip is correct because you need a cover slip to cover the specimen on the slide. A cover slip is a thin, transparent piece of glass or plastic that can be placed over the specimen on the slide. A cover slip protects the specimen from drying out, dust, or damage, and creates a flat surface for viewing under the microscope.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
Choice A rationale: H2O is a reactant or input for the light reaction of photosynthesis. In the light reaction, water is split by the energy from sunlight in photosystem II, releasing electrons, protons, and oxygen. The electrons and protons are used to reduce NADP+ to NADPH and to synthesize ATP from ADP and Pi. The oxygen is either used for respiration or released into the air¹².
Choice B rationale: Oxygen is not a reactant or input for the light reaction of photosynthesis, but a product or output. Oxygen is released as a by-product of the splitting of water in photosystem II. Oxygen is either used for respiration or released into the air¹².
Choice C rationale: NADPH is not a reactant or input for the light reaction of photosynthesis, but a product or output. NADPH is an electron carrier that is reduced by the electrons from water in photosystem I. NADPH provides electrons and hydrogen for the dark reaction, which uses CO2 to produce glucose¹².
Choice D rationale: ATP is not a reactant or input for the light reaction of photosynthesis, but a product or output. ATP is an energy molecule that is synthesized by the enzyme ATP synthase using the proton gradient created by the electron transport chain. ATP provides energy for the dark reaction, which uses CO2 to produce glucose¹².
Choice E rationale: Carbon dioxide is not a reactant or input for the light reaction of photosynthesis, but a reactant or input for the dark reaction. The dark reaction uses CO2 and energy intermediates from the light reaction to produce glucose, a type of sugar. The dark reaction does not require light and can occur in the day or night¹².
Correct Answer is A
Explanation
Choice A rationale: Iodine is a chemical element that forms a brown solution of iodine and potassium iodide, known as iodine solution. When this solution is added to a sample that contains starch, it forms a complex with the starch molecules, which changes the color of the solution to blue-black. This is based on the fact that starch is a polysaccharide that has a helical structure, which can trap the iodine molecules inside. ²
Choice B rationale: Biuret is a chemical compound that forms a blue solution of copper (II) sulfate and sodium hydroxide, known as biuret reagent. When this reagent is added to a sample that contains proteins or peptides, it forms a complex with the copper (II) ions, which changes the color of the solution to violet or pink. This is based on the fact that proteins and peptides have peptide bonds, which have nitrogen atoms that can coordinate with the copper (II) ions. ³
Choice C rationale: Benedict's is a chemical compound that forms a blue solution of copper (II) sulfate, sodium carbonate, and sodium citrate, known as Benedict's reagent. When this reagent is heated with a sample that contains reducing sugars, such as glucose or maltose, it reduces the copper (II) ions to copper (I) ions, which form a red, orange, or green precipitate of copper (I) oxide. This is based on the fact that reducing sugars have free aldehyde or ketone groups that can donate electrons to the copper (II) ions. ⁴
Choice D rationale: Phenol red is a chemical compound that forms a red solution that is used as a pH indicator. When this solution is added to a sample that has an acidic or neutral pH, it remains red or turns yellow. When this solution is added to a sample that has an alkaline pH, it turns pink or fuchsia. This is based on the fact that phenol red has a sulfonated hydroxyquinone group that can lose or gain protons depending on the pH of the solution. ⁵
Choice E rationale: Sudan IV is a chemical compound that forms a red powder that is used as a stain for lipids. When this powder is dissolved in a solvent and added to a sample that contains lipids, such as fats or oils, it dissolves in the lipids and stains them red. When this solution is added to a sample that does not contain lipids, it remains in the solvent and does not stain the sample. This is based on the fact that Sudan IV is a nonpolar compound that can dissolve in nonpolar substances like lipids, but not in polar substances like water. ⁶.
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