What is a primer in DNA sequencing?
A short piece of double-stranded DNA that binds to the template DNA and acts as a "starter" for the polymerase.
A short piece of double-stranded DNA that binds to the primer and acts as a "starter" for the template.
A short piece of single-stranded DNA that binds to the template DNA and acts as a "starter" for the polymerase.
A short piece of single-stranded DNA that binds to the polymerase and acts as a "starter" for the template.
The Correct Answer is C
A primer is a short single-stranded DNA fragment used in certain laboratory techniques, such as the polymerase chain reaction (PCR).
In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified.
Choice A) A short piece of double-stranded DNA that binds to the template DNA and acts as a “starter” for the polymerase is incorrect because primers are single-stranded, not double-stranded.
Choice B) A short piece of double-stranded DNA that binds to the primer and acts as a “starter” for the template is incorrect because it does not make sense for a primer to bind to itself.
Choice D) A short piece of single-stranded DNA that binds to the polymerase and acts as a “starter” for the template is incorrect because primers bind to the template DNA, not to the polymerase.
Nursing Test Bank
Naxlex Comprehensive Predictor Exams
Related Questions
Correct Answer is A
Explanation
The body regulates blood pH through several mechanisms, including chemical buffers, the respiratory system, and the urinary system.
The respiratory system can adjust blood pH by changing the rate of respiration to remove or retain CO2.
When there is excess acid in the blood, the respiratory rate increases to remove more CO2, which helps to raise blood pH.
Choice B is incorrect because decreasing the respiration rate would retain CO2, which would lower blood pH.
Choice C is incorrect because increased water intake would not directly affect blood pH levels.
Choice D is incorrect because decreased water intake would not directly affect blood pH levels.
Correct Answer is D
Explanation
Genes that regulate cell division can become oncogenes when mutated.
Oncogenes are mutated genes that can contribute to the development of cancer.
In their non-mutated state, everyone has genes which are referred to as proto-oncogenes.
When proto-oncogenes are mutated or increased in numbers due to DNA damage, the proteins produced by these genes can affect the growth, proliferation, and survival of the cell, and potentially result in the formation of a malignant tumor.
Choice A is incorrect because mutations in oncogenes do not always result in the inhibition of cell division.
Instead, they can contribute to the development of cancer by affecting cell growth.
Choice B is incorrect because oncogenes are not only found in human cells but can be present in other organisms as well.
Choice C is incorrect because genes that regulate cell division can be found in viruses.
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