What is a primer in DNA sequencing?
A short piece of double-stranded DNA that binds to the template DNA and acts as a "starter" for the polymerase.
A short piece of double-stranded DNA that binds to the primer and acts as a "starter" for the template.
A short piece of single-stranded DNA that binds to the template DNA and acts as a "starter" for the polymerase.
A short piece of single-stranded DNA that binds to the polymerase and acts as a "starter" for the template.
Correct Answer : C
A primer is a short single-stranded DNA fragment used in certain laboratory techniques, such as the polymerase chain reaction (PCR).
In the PCR method, a pair of primers hybridizes with the sample DNA and defines the region that will be amplified.
Choice A) A short piece of double-stranded DNA that binds to the template DNA and acts as a “starter” for the polymerase is incorrect because primers are single-stranded, not double-stranded.
Choice B) A short piece of double-stranded DNA that binds to the primer and acts as a “starter” for the template is incorrect because it does not make sense for a primer to bind to itself.
Choice D) A short piece of single-stranded DNA that binds to the polymerase and acts as a “starter” for the template is incorrect because primers bind to the template DNA, not to the polymerase.
Note: DNA primers are used instead of RNA primers in DNA sequencing and PCR because DNA is more stable, specific, and compatible with the enzymes and processes involved in these techniques.
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Correct Answer is A
Explanation
Corpus luteum.
During the menstrual cycle, the corpus luteum in the ovary produces progesterone to prepare the endometrium for potential implantation.
Choice B is incorrect because fimbriae are finger-like projections at the end of the fallopian tubes that help guide the egg into the tube.
Choice C is incorrect because a follicle is a sac in the ovary that contains an immature egg.
Choice D is incorrect because the ovarian ligament is a fibrous band of tissue that connects the ovary to the uterus.
Correct Answer is A
Explanation
Pattern recognition receptors (PRRs) are a class of receptors that can directly recognize the specific molecular structures on the surface of pathogens.
PRRs play a crucial role in the proper function of the innate immune system and are germline-encoded host sensors, which detect molecules typical for the pathogens.
Choice B is incorrect because cytokines are not receptors but rather signaling molecules that regulate immunity.
Choice C is incorrect because chemokines are not receptors but rather signaling molecules that attract immune cells to sites of infection.
Choice D is incorrect because T cells are not receptors but rather white blood cells that assist B cells or directly kill infected cells.
Correct Answer is A
Explanation
Hydrogen bonding is an interaction involving a hydrogen atom located between a pair of other atoms having a high affinity for electrons.
One atom of the pair (the donor), generally a fluorine, nitrogen, or oxygen atom, is covalently bonded to a hydrogen atom, whose electrons it shares unequally; its high electron affinity causes the hydrogen to take on a slight positive charge.
The other atom of the pair (the acceptor), also typically F, N, or O, has an unshared electron pair, which gives it a slight negative charge.
Mainly through electrostatic attraction, the donor atom effectively shares its hydrogen with the acceptor atom, forming a bond.
Choice B) The repulsion between the positive and negative charges of two molecules is incorrect because hydrogen bonding involves attraction, not repulsion.
Choice C) The attraction between two nonpolar molecules is incorrect because hydrogen bonding involves polar molecules.
Choice D) The attraction between two ionic molecules is incorrect because hydrogen bonding involves polar molecules and not ionic molecules.
Correct Answer is A
Explanation
The correct answer is choice A.
The spleen is an organ in the human body that is responsible for the removal of damaged red blood cells and the production of certain types of white blood cells.
Choice B is incorrect because the kidneys are responsible for filtering waste from the blood and regulating electrolyte balance.
Choice C is incorrect because the pancreas produces hormones and enzymes that aid in digestion.
Choice D is incorrect because the thyroid gland produces hormones that regulate metabolism.
Correct Answer is B
Explanation
The correct answer is choice B.
To amplify specific regions of DNA.
PCR (polymerase chain reaction) is a laboratory technique used to make many copies of a specific region of DNA.
The goal of PCR is to make enough of the target DNA region that it can be analyzed or used in some other way.
PCR has many research and practical applications, including DNA cloning, medical diagnostics, and forensic analysis of DNA.
Choice A is incorrect because PCR does not separate DNA fragments by size. Choice C is incorrect because PCR does not sequence DNA fragments.
Choice D is incorrect because PCR does not analyze protein expression levels.
Correct Answer is B
Explanation
The correct answer is choice B.
Maintaining normal body temperature and blood pressure.
Early identification and management of potential organ donors must take into consideration specific pathophysiologic changes for medical optimization 1.
The VIPPS (ventilation, infusion and pumping, pharmacological treatment, and specificities) strategy is a mnemonic method that brings together key aspects of the restoration of oxygen delivery to tissues during hemodynamic instability plus organ optimization strategies.
Choice A is incorrect because administering antibiotics to prevent infection is not the most important intervention to preserve organ viability.
Choice C is incorrect because providing emotional support to family members, while important, is not an intervention that directly affects organ viability.
Choice D is incorrect because applying eye drops and ointment to prevent corneal drying is not the most important intervention to preserve organ viability.
Correct Answer is A
Explanation
The correct answer is choice A. Keratin.
Keratin is a fibrous protein that provides strength and protection to the body, particularly in the skin, hair, and nails.
It is found in epithelial tissue, which covers the body’s surface and lines its internal organs and cavities.
Choice B.
Collagen is incorrect because collagen is a fibrous protein that provides strength and support to connective tissues such as tendons, ligaments, and cartilage.
Choice C.
Elastin is incorrect because elastin is a protein that provides elasticity to tissues such as skin and blood vessels.
Choice D.
Actin is incorrect because actin is a protein that plays a role in muscle contraction and cell movement.
Correct Answer is A
Explanation
In a well-designed experiment, all variables apart from the treatment should be kept constant between the control group and treatment group.
This means researchers can correctly measure the entire effect of the treatment without interference from confounding variables.
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Choice B) Independent variable and dependent variable is incorrect because these are not groups but rather variables.
The independent variable is manipulated by the experimenters while the dependent variable is measured to see if it changes as a result of the manipulation.
Choice C) Experimental group and non-experimental group is incorrect because a non-experimental group is not a term used in experimental design.
The correct term for the group that does not receive the treatment is control group.
Choice D) High level and low level of the independent variable is incorrect because these are levels of the independent variable, not groups.
In an experiment, there can be multiple levels of the independent variable, but they are applied to different groups (e.g.
control group, treatment group).
Correct Answer is C
Explanation
The correct answer is choice C. Thyroxine.
Thyroxine (T4) is a hormone produced by the thyroid gland that controls your body’s metabolism, the process in which your body transforms the food you eat into energy.
Choice A, Estrogen, is not the correct answer because it is a hormone responsible for the development of female secondary sexual characteristics.
Choice B, Progestin, is not the correct answer because it is a synthetic form of progesterone used in hormonal birth control and hormone replacement therapy.
Choice D, Androgen, is not the correct answer because it is a hormone responsible for the development of male secondary sexual characteristics.
Correct Answer is B
Explanation
The correct answer is choice B.
Using a placebo group and a double-blind technique for giving the medications is the best way to ensure that the study is valid and reliable.
A placebo group helps control for the placebo effect, which can influence the results of a study.
A double-blind technique means that neither the patients nor the researchers know which medication is being given, reducing bias.
Choice A is not the best answer because while a large sample size and standardized procedure can increase reliability, they do not address validity.
Choice C is not the best answer because a matched-pairs design and crossover technique are useful for reducing variability but do not address validity.
Choice D is not the best answer because a convenience sample may not be representative and a pretest-posttest design does not control for extraneous variables.
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