To separate genomic DNA fragments by size, which of these laboratory methods is most useful?
Filtration
Spectrophotometry
Titration
Electrophoresis
Correct Answer : D
One of the most common techniques for separating DNA fragments by size is electrophoresis. Electrophoresis is a process that uses an electric field to move charged molecules through a porous gel matrix. The gel acts as a sieve, allowing smaller molecules to move faster and farther than larger ones. By applying a dye to the DNA samples and loading them into wells at one end of the gel, the DNA fragments can be visualized as bands after electrophoresis. The distance of each band from the well indicates the size of the corresponding DNA fragment.
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Correct Answer is B
Explanation
Triple covalent bonds. Nitrogen gas consists of two nitrogen atoms that share three pairs of electrons, forming a triple bond. This bond is very strong and requires a lot of energy to break. Ionic bonds, resonance bonds, and hydrogen bonds are weaker types of bonds that do not occur in nitrogen gas.
Correct Answer is D
Explanation
One of the most common techniques for separating DNA fragments by size is electrophoresis. Electrophoresis is a process that uses an electric field to move charged molecules through a porous gel matrix. The gel acts as a sieve, allowing smaller molecules to move faster and farther than larger ones. By applying a dye to the DNA samples and loading them into wells at one end of the gel, the DNA fragments can be visualized as bands after electrophoresis. The distance of each band from the well indicates the size of the corresponding DNA fragment.
Correct Answer is B
Explanation
A buffer is a solution that can resist changes in pH when small amounts of acid or base are added. In the human body, blood is an important buffer that helps maintain a stable pH of about 7.4. One of the main components of the blood buffer system is the bicarbonate buffer, which involves the equilibrium between carbon dioxide (CO2), carbonic acid (H2CO3), and bicarbonate ions (HCO3-). When blood pH rises, meaning it becomes more basic, the bicarbonate buffer can donate H+ ions to lower the pH and restore the balance. The substance that donates H+ ions in this case is carbonic acid, which dissociates into H+ and HCO3-. Therefore,
Correct Answer is C
Explanation
Fungi are eukaryotic organisms, meaning they have a defined nucleus and membrane-bound organelles, including mitochondria. Additionally, fungal cells have a cell wall made primarily of chitin, which is a distinguishing characteristic from plants that have cell walls made of cellulose.
Under a microscope, fungal cells may appear as:
- Yeast: Single-celled fungi, which are oval or round and reproduce by budding.
- Molds: Multicellular fungi with filamentous structures called hyphae that form a network known as mycelium.
Why the Other Options Are Incorrect:
- A. A chain of rod-shaped cells without a defined nucleus: This describes bacteria, which are prokaryotic cells without a nucleus or membrane-bound organelles.
- B. A single-celled organism with chloroplasts and a defined nucleus: This describes a plant cell or algae, specifically a photosynthetic organism. Fungi do not have chloroplasts and cannot perform photosynthesis.
- D. A single-celled organism with a defined nucleus and pseudopodia: This describes a protozoan, such as an amoeba. Pseudopodia are extensions of the cell membrane used for movement and feeding, which are not characteristic of fungi.
Therefore, the most likely microscopic observation of a fungal microbe would be an organism with mitochondria, cell walls, and a nucleus.
Correct Answer is B
Explanation
The process in which an ovarian follicle matures and releases a reproductive egg is called ovulation. Ovulation is one of the phases of the menstrual cycle, which also includes follicular phase, luteal phase and menstruation
Correct Answer is A
Explanation
One of the ways that cells regulate their water balance is by adjusting to different solute concentrations in their environment. When a cell is placed in a solution that has a higher concentration of solutes than the cell itself, the solution is called hypertonic. In this case, water will tend to move out of the cell through special channels called aquaporins, which are embedded in the plasma membrane. This movement of water across a selectively permeable membrane is a type of passive transport called osmosis. Osmosis does not require any energy input from the cell, unlike other forms of transport such as diffusion, facilitated diffusion, or active transport.
Correct Answer is C
Explanation
When a muscle cell is stimulated by a nerve impulse, calcium ions are released from the sarcoplasmic reticulum into the cytoplasm. The calcium ions bind to the troponin complex, which is a protein that regulates the interaction between actin and myosin filaments. The binding of calcium to troponin causes a conformational change that exposes the binding sites for myosin on the actin filaments. This allows the myosin heads to attach to the actin and pull the filaments past each other, resulting in muscle contraction.
Correct Answer is B
Explanation
The number of protons in an atom is equal to its atomic number. In this case, lithium has an atomic number of 3, so it contains 3 protons.
The other choices (A, C, and D) do not represent the correct number of protons for a lithium atom.
Correct Answer is C
Explanation
Antidiuretic hormone (ADH) is a peptide hormone that regulates the water balance in the body by increasing the reabsorption of water by the kidneys. ADH is synthesized by the hypothalamus, a part of the brain that controls many functions, such as appetite, sleep, and body temperature. ADH is then stored and released by the posterior pituitary gland, which is located at the base of the brain.
Correct Answer is D
Explanation
The experimental group is the group that receives the treatment or intervention in a scientific study. To evaluate the effect of the treatment, the experimental group is compared to another group that does not receive the treatment. This group is called the control group. The control group serves as a baseline or reference point for the experimental group. The control group should be as similar as possible to the experimental group in all other aspects, except for the treatment
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